CD22 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Aliases
SIGLEC2, SIGLEC-2
Gene ID
933
Swiss Prot
P20273
Reactivity
Human
Immunogen
Raji (Burkitt's lymphoma) cells.
Target
Monoclonal Mouse Anti-Human CD22
Clonality
Monoclonal
Clone
B456 (FR10B4)
Type
Monoclonal Antibody
Applications
IHC-F, FC
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Format
Liquid.// 20mM Tris-borate, 150mM NaCl, RPMI 1640/D-MEM contaiing fetal bovine serum, GMC-6 carrier polysaccharides, carrier protein and 0.05% sodium azide, pH 7.5
Reconstitution
Store at 2 - 8C for short term, freeze under -20C for long term storage.
Fragment
IgG1
Specificity
This antibody reacts strongly with CD22. CD22 is expressed on in all B cell cytoplasm. It appears as CD19 on the cell surface in developing B cells. It can also be found on the cell surface of most mature sIg+ B cells and on hairy cell and prolymphocytic leukemia cells. On frozen sections, CD22 is found in follicular mantle and marginal zone B cells. There is weak reaction in germinal center B cells. This antibody can be used for typing of leukemia, assigning B cell lineage, and in flow cytometry to identify mature B cells. Also, it can be used to study B cell adhesion and to identify B cell lymphomas.
NCBI Gene Symbol
CD22
Host or Source
Mouse
Protein Name
B-cell receptor CD22
Gene Name URL
CD22
Frequently Asked Questions
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