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CD22 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Aliases

SIGLEC2, SIGLEC-2

Gene ID

933

Swiss Prot

P20273

Reactivity

Human

Immunogen

Raji (Burkitt's lymphoma) cells.

Target

Monoclonal Mouse Anti-Human CD22

Clonality

Monoclonal

Clone

B456 (FR10B4)

Type

Monoclonal Antibody

Applications

IHC-F, FC

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Liquid.// 20mM Tris-borate, 150mM NaCl, RPMI 1640/D-MEM contaiing fetal bovine serum, GMC-6 carrier polysaccharides, carrier protein and 0.05% sodium azide, pH 7.5

Reconstitution

Store at 2 - 8C for short term, freeze under -20C for long term storage.

Fragment

IgG1

Specificity

This antibody reacts strongly with CD22. CD22 is expressed on in all B cell cytoplasm. It appears as CD19 on the cell surface in developing B cells. It can also be found on the cell surface of most mature sIg+ B cells and on hairy cell and prolymphocytic leukemia cells. On frozen sections, CD22 is found in follicular mantle and marginal zone B cells. There is weak reaction in germinal center B cells. This antibody can be used for typing of leukemia, assigning B cell lineage, and in flow cytometry to identify mature B cells. Also, it can be used to study B cell adhesion and to identify B cell lymphomas.

NCBI Gene Symbol

CD22

Host or Source

Mouse

Protein Name

B-cell receptor CD22

Gene Name URL

CD22

Frequently Asked Questions

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