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POU5F1 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Aliases

S, gp, po, gp-, gp9, oct, spg, gp-9, oct4, pou2, pou5, POU-2, cb197, pou5f1, chunp68, spg/pou2, chunp6868, wu:fd18d06, etID49452.21

Gene ID

30333

Swiss Prot

Q90270

Reactivity

Zebrafish

Immunogen

This DANRE pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 128-161 amino acids from the N-terminal region of human DANRE pou5f1.

Target

Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Reconstitution

Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

52 kDa

Fragment

Rabbit Ig

NCBI Gene Symbol

POU5F1

Host or Source

Rabbit

Gene Name URL

POU5F1

Frequently Asked Questions

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