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ART3 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Aliases

ARTC3

Gene ID

419

Swiss Prot

Q13508

Accession Number

NP_001123488.1

Reactivity

Human, Mouse

Immunogen

This ART3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human ART3.

Target

Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI) -anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

2 mg/ml

Format

Liquid// PBS with 0.09% (W/V) sodium azide.

Reconstitution

Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

44 kDa

Fragment

Ig

NCBI Gene Symbol

ART3

Host or Source

Rabbit

Protein Name

Ecto-ADP-ribosyltransferase 3

Gene Name URL

ART3

Frequently Asked Questions

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