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IL-27 Antibody - middle region

IL-27 is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent.

Product Specifications

Gene Name

Interleukin 17D

Gene Aliases

IL-17D; interleukin-17D; Interleukin-27.

Gene ID

53342

Swiss Prot

https://www.uniprot.org/uniprot/Q8TAD2

Accession Number

https://www.ncbi.nlm.nih.gov/protein/NP_612141.1

Reactivity

Human, Mouse

Immunogen

This IL-27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-87 amino acids from the Central region of human IL-27.

Target

Interleukin-17D, interleukin-17D precursor [Homo sapiens]

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Flow cytometry, Immunohistochemistry-Paraffin, Western blot

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

Varies by lot. See vial for concentration.

Format

Liquid// PBS with 0.09% (W/V) sodium azide.

Reconstitution

Maintain refrigerated at 2-8° C for up to 2 weeks. For long term storage store at -20° C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

21893

Fragment

Ig

NCBI Gene Symbol

IL17D

Host or Source

Rabbit

Protein Name

NP_612141.1, Q8TAD2

Gene Name URL

IL17D

CAS Number

9007-83-4

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