SEMA6D Antibody - N-terminal region
Product Specifications
CAS Number
9007-83-4
Gene Aliases
SEMA6D, KIAA1479, Semaphorin-6D
Gene ID
80031
Swiss Prot
Q8NFY4
Accession Number
NP_001185928.1
Reactivity
Human, Mouse
Immunogen
This SEMA6D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from the N-terminal region of human SEMA6D.
Target
Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Format
Liquid. PBS with 0.09% (W/V) sodium azide.
Reconstitution
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
119872 Da
Fragment
Ig
NCBI Gene Symbol
SEMA6D
Host or Source
Rabbit
Protein Name
Semaphorin-6D
Gene Name URL
SEMA6D
Frequently Asked Questions
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