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PLOD1 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Aliases

LH, LH1, LLH, EDS6, PLOD, EDSKCL1

Gene ID

5351

Swiss Prot

Q02809

Accession Number

NP_000293.2

Reactivity

Human

Immunogen

This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1.

Target

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC-P, FC

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

Approximately 0.5mg/ml. Actual concentration varies with each lot.

Homology

Mouse

Format

Liquid// PBS with 0.09% (W/V) sodium azide.

Reconstitution

Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

84 kDa

Fragment

Ig

NCBI Gene Symbol

PLOD1

Host or Source

Rabbit

Protein Name

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene Name URL

PLOD1

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