PLOD1 Antibody - N-terminal region
Product Specifications
CAS Number
9007-83-4
Gene Aliases
LH, LH1, LLH, EDS6, PLOD, EDSKCL1
Gene ID
5351
Swiss Prot
Q02809
Accession Number
NP_000293.2
Reactivity
Human
Immunogen
This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1.
Target
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IHC-P, FC
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Homology
Mouse
Format
Liquid// PBS with 0.09% (W/V) sodium azide.
Reconstitution
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
84 kDa
Fragment
Ig
NCBI Gene Symbol
PLOD1
Host or Source
Rabbit
Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Gene Name URL
PLOD1
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