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EEF1A1 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Aliases

CCS3, EF1A, PTI1, CCS-3, EE1A1, EEF-1, EEF1A, EF-Tu, LENG7, eEF1A-1, GRAF-1EF

Gene ID

1915

Swiss Prot

P68104

Accession Number

NP_001393.1

Reactivity

Human

Immunogen

This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids of human EEF1A1.

Target

EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC-P, FC

Purification

This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

2 mg/ml

Homology

Xenopus, Bovine, Hamster, Drosophila, Horse, Mouse, Rabbit, Rat, C.Elegans, Chicken, Zebrafish, Yeast

Format

Liquid.// PBS with 0.09% (W/V) sodium azide.

Reconstitution

Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

50 kDa

Fragment

Rabbit Ig

NCBI Gene Symbol

EEF1A1

Host or Source

Rabbit

Protein Name

Elongation factor 1-alpha 1

Gene Name URL

EEF1A1

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