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MDM2 Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

MDM2 proto-oncogene

Gene Aliases

HDMX, LSKB, hdm2, ACTFS

Gene ID

4193

Swiss Prot

Q00987-8

Accession Number

NP_001138811.1

Reactivity

Human

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human MDM2

Target

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

KSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGD

Applications

WB

Purification

Affinity purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

55 kDa

Protein Length

491

NCBI Gene Symbol

MDM2

Host or Source

Rabbit

Protein Name

E3 ubiquitin-protein ligase Mdm2

Gene Name URL

MDM2

Nucleotide Accession Number

NM_001145339.2

Frequently Asked Questions

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