Anti-TSG101 Antibody

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). {PubMed:11916981, PubMed:17556548, PubMed:17853893, PubMed:21070952, PubMed:21757351, PubMed:22315426, PubMed:22660413}.

ELISA: use at 1-5ug/ml. <br><br>Immunoblotting: use at a 1:500-1:3,000 dilution. A band of 46kDa is detected. Detection of TSG101 protein in (A) NIH-3T3 cell lysate, (B) JC cell lysate, and (C) BCL-1 cell lysate with #3352 diluted 1:500. <br><br>Endusers should determine optimal antibody concentrations for their applications. <br><br>IHC: use at a dilution of 1:100 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of TSG101 protein in paraffin-embedded human breast carcinoma. Detection of TSG101 protein in paraffin-embedded human ovarian carcinoma.