MRPL14 Antibody - C-terminal region

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Product Specifications

CAS Number

9007-83-4

Gene Aliases

L14mt, L32mt, MRPL32, RMPL32, RPML32, MRP-L14, MRP-L32

Gene ID

64928

Swiss Prot

Q6P1L8

Accession Number

NP_115487

Reactivity

Human, Mouse, Rat, Cow, Dog, Goat, Horse, Pig, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the C-terminal region of Human MRPL14

Target

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3.

Partner Proteins

RPS20; NDUFA7; MRPL53; GADD45GIP1; SARNP; MRPL38; MRPL40; MRPS14; SRPRB; CDV3; MRPS10; MRPL16; MRPL39; MRPL4; MRPL2; MRPL22; MRPL15; MRPL13; HNRNPR; PPIF; MRPL19; EEF1A1; DUT; TUFM; CUL3; UBC; ICT1

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

MTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRKREGEYSKVLAIAQNFV

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 86%; Dog: 86%; Goat: 86%; Horse: 86%; Human: 100%; Mouse: 93%; Pig: 79%; Rat: 93%; Zebrafish: 85%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

15kDa

Protein Length

145

NCBI Gene Symbol

MRPL14

Host or Source

Rabbit

Protein Name

39S ribosomal protein L14, mitochondrial

Gene Name URL

MRPL14

Nucleotide Accession Number

NM_032111

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