COBRA1 Antibody - N-terminal region (ARP65310_P050)

Product Specifications

CAS Number

9007-83-4

Gene Name

Negative elongation factor complex member B

Gene Aliases

COBRA1, NELF-B

Gene ID

25920

Swiss Prot

Q8WX92

Accession Number

NP_056271

Reactivity

Human

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human COBRA1

Target

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040) . NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]) .[supplied by OMIM, Mar 2008]

Partner Proteins

UBC; ABCF1; DIMT1; EDC4; IGF2BP3; SYNCRIP; EIF2B2; EIF2B3; EIF3C; YBX3; RPL23A; RFC4; PTBP1; PA2G4; YBX1; NMT1; MRE11A; ILF2; HNRNPU; FKBP3; DHX9; DDX1; KHDRBS3; DENR; YWHAB; MGST1; HSPA9; Dlg4; POLR2C; NELFB; SUPT5H; POLR2A; ATP1A1; ALDH3A2; NELFCD; CCT8

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

NCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRD

Applications

Purification

Affinity purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Yeast: 90%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

66 kDa

Protein Length

580

NCBI Gene Symbol

NELFB

Host or Source

Rabbit

Protein Name

Negative elongation factor B

Gene Name URL

NELFB

Nucleotide Accession Number

NM_015456.4

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