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EIF3F Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

Eukaryotic translation initiation factor 3, subunit F

Gene Aliases

MRT67, EIF3S5, eIF3-p47

Gene ID

8665

Swiss Prot

O00303

Accession Number

NP_003745

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Target

EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Partner Proteins

HUWE1; UBC; SUMO2; RNF2; FBXO6; RPS6KB1; IGSF8; ICAM1; CD81; DDX3X; gag-pol; EIF3D; EIF3B; ATXN1; SMAD9; FN1; EIF3M; EIF3C; EIF3A; MSMO1; EIF3E; ATP6V1A; APP; EIF3L; EIF3K; MRPL46; EIF3I; EIF3H; EIF3G; UBD; HNRNPK; CDK11B; YWHAZ; NOTCH1; DTX1; RAD21; FBXO

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

VILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEV

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

39kDa

Protein Length

357

NCBI Gene Symbol

EIF3F

Host or Source

Rabbit

Protein Name

Eukaryotic translation initiation factor 3 subunit F

Gene Name URL

EIF3F

Nucleotide Accession Number

NM_003754

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