EIF5B Antibody - middle region
Product Specifications
CAS Number
9007-83-4
Gene Name
Eukaryotic translation initiation factor 5B
Gene Aliases
IF2
Gene ID
9669
Swiss Prot
O60841
Accession Number
NP_056988
Reactivity
Human, Cow, Dog, Horse, Pig, Rabbit
Target
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3) . Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
Partner Proteins
TP53; TFCP2; UBC; LGR4; YBX3; ETS1; MMS19; FN1; CSNK2A1; PAXIP1; ECT2; BRCA1; EIF3C; CAND1; YWHAZ; SIRT7; HDGF; Mapk13; POU5F1; ZBTB3; PRKAA1; EIF1AX; CBL; gag
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
SEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAY
Applications
WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Cow: 86%; Dog: 86%; Horse: 86%; Human: 100%; Pig: 93%; Rabbit: 93%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
139kDa
Protein Length
1220
NCBI Gene Symbol
EIF5B
Host or Source
Rabbit
Protein Name
Eukaryotic translation initiation factor 5B
Gene Name URL
EIF5B
Nucleotide Accession Number
NM_015904
Curated Selection
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