H3f3a Antibody - N-terminal region
Product Specifications
CAS Number
9007-83-4
Gene Name
H3 histone, family 3A
Gene Aliases
H3-3, H3.3, H3-3a, H3-3b, H3.3A, EyeLinc14
Gene ID
15078
Swiss Prot
P84244
Accession Number
NP_032236
Reactivity
Rat, Dog, Pig, Zebrafish
Target
H3f3a is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. H3f3a constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. H3f3a is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Partner Proteins
Kmt2d; Chd5; Setd1a; Kmt2c; Setd1b; Ezh2; Chd1; Cbx3; Uhrf1; Nfkb2; Hdac5; Hdac3; Kdm6b; Nfe2; Aurka; Cbx2; Ehmt2; Cbx5; Aurkb; Cbx6; Bptf; Creb3l4; Ing2; Cbx7; Cbx8; Cbx4; Cd4; Fosb; Rps6ka5; Rps6ka3; Igh-8; Cd79a; Tcrb; Eif2s3x; Kdm5c; Nanog; Kcnq1ot1
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
KSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQ
Applications
WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Dog: 100%; Pig: 100%; Rat: 100%; Zebrafish: 100%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
15kDa
Protein Length
126
NCBI Gene Symbol
H3F3A
Host or Source
Rabbit
Protein Name
Histone H3.3
Gene Name URL
H3f3a
Nucleotide Accession Number
NM_008210
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