CDC27 Antibody - C-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

Cell division cycle 27

Gene Aliases

APC3, HNUC, NUC2, H-NUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E

Gene ID

996

Swiss Prot

P30260

Accession Number

NP_001107563.1

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the C-terminal region of human CDC27

Target

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

SYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSF

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

37kDa

Protein Length

343

NCBI Gene Symbol

CDC27

Host or Source

Rabbit

Protein Name

Cell division cycle protein 27 homolog

Gene Name URL

CDC27

Nucleotide Accession Number

NM_001114091.2

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

TR707193 1 Kit

Ccl19 Rat shRNA Plasmid (Locus ID 362506)

Ask

View Details

E-HD-C085 20 Tests

Human HP (Urease/CagA/VacA) Antibody Lateral Flow Assay Kit

Ask

View Details

E28PN7919 100 µL

ATOX1 Rabbit Polyclonal Antibody

Ask

View Details

40669111 3x 1.0 µg

RNY5P9 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

Ask

View Details

GTR15326687 1 Vial

Lentiviral human AKR1E2 shRNA (UAS) - Lentiviral human AKR1E2 shRNA (UAS, GFP) (25)

Ask

View Details

036672-ML750 100 µL

HNRPLL, NT (HNRPLL, SRRF, Heterogeneous nuclear ribonucleoprotein L-like, Stromal RNA-regulating factor) (MaxLight 750) discontinued

Ask

View Details