PSMF1 Antibody - middle region
Product Specifications
CAS Number
9007-83-4
Gene Name
Proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
Gene Aliases
PI31
Gene ID
9491
Swiss Prot
Q92530
Accession Number
NP_006805
Reactivity
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human PSMF1
Target
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.
Partner Proteins
FBXO7; SHFM1; UBC; BEND7; RHOXF2; KHDRBS3; PAK7; LDOC1; RBFOX2; PSMA7; DVL3; RALYL; RBFOX1; QKI; CD2BP2; CUL1; PSMA2; MIEF2; CCDC85B; RNF126; PDLIM7; HOOK2; NUDT21; CTBP2; TRAF2; MAGEA11; RBMX
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
DYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFP
Applications
WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
30kDa
Protein Length
271
NCBI Gene Symbol
PSMF1
Host or Source
Rabbit
Protein Name
Proteasome inhibitor PI31 subunit
Gene Name URL
PSMF1
Nucleotide Accession Number
NM_006814
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