WDR77 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

WD repeat domain 77

Gene Aliases

P44, MEP50, MEP-50, HKMT1069, Nbla10071, p44/Mep50

Gene ID

79084

Swiss Prot

Q9BQA1

Accession Number

NP_077007

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human WDR77

Target

WDR77 is the non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. WDR77 might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

Partner Proteins

TP53; CUL3; SMAD1; AR; UBC; ASB15; WWOX; ZBTB1; SMYD1; COPS4; COPS7A; DCAF8; COPS8; PRMT5; TBC1D4; EPB41L2; HGS; FBXO6; PAN2; SNRPC; PPP1R8; POLR2C; PCBP2; HNRNPM; GRB2; QKI; TOE1; PPIL1; SAP30BP; LSM3; GPKOW; SF3A1; SF3B4; RBM5; PQBP1; THOC1; AQR; BAG2

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLS

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol