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BUB3 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

Budding uninhibited by benzimidazoles 3 homolog (yeast)

Gene Aliases

BUB3L, hBUB3

Gene ID

9184

Swiss Prot

A6NJ42

Accession Number

NP_001007794

Reactivity

Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human BUB3

Target

BUB3 is required for kinetochore localization of BUB1.This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Partner Proteins

BUB1B; BUB1; HUWE1; PSTPIP1; UBC; SUMO2; CDC20; NEDD8; SUMO1; RPA3; RPA2; RPA1; WBP4; CLIP1; EGFR; ZNF207; VCAM1; UBA1; THRA; RPL37A; ITGA4; FN1; NKAP; EXOC3; SUGT1; CPNE1; ANAPC4; SEPT2; TOP1; SMAD3; SMAD2; Mad2l1; COPS5; CUL3; SF3A2; ELAVL1; CDC27; HDGF

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMR

Applications

WB, IF, IHC

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

36kDa

Protein Length

326

NCBI Gene Symbol

BUB3

Host or Source

Rabbit

Protein Name

Mitotic checkpoint protein BUB3

Gene Name URL

BUB3

Nucleotide Accession Number

NM_001007793

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