TRIM25 antibody - middle region (ARP58106_P050)
Product Specifications
Gene Name
Tripartite motif containing 25
Gene Aliases
EFP, Z147, RNF147, ZNF147
Gene ID
7706
Swiss Prot
Q14258
Accession Number
NP_005073
Reactivity
Human, Cow, Pig
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human TRIM25
Target
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Partner Proteins
UBC; DDX58; SUMO2; AMFR; UBE2D1; SUMO1; PCNA; USP15; UBA1; RPS27A; RPA1; KIF5B; HSPA1L; HSPA1B; HSPA1A; EEF1A2; ACLY; SUZ12; RNF2; EZH2; BMI1; HECW2; TRIM25; TRIM8; TFG; GRIK2; ESR1; OTUB2; USP39; VCP; WARS; UAP1; UBXN6; UBE2S; UBE2D2; TRAF6; ISG15; UBE2D
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
TPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGA
Applications
WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Cow: 91%; Human: 100%; Pig: 79%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
71kDa
Protein Length
630
NCBI Gene Symbol
TRIM25
Host or Source
Rabbit
Protein Name
E3 ubiquitin/ISG15 ligase TRIM25
Gene Name URL
TRIM25
CAS Number
9007-83-4
Nucleotide Accession Number
NM_005082
Curated Selection
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