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PKM2 antibody - middle region (ARP57789_P050)

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Product Specifications

Gene Name

Pyruvate kinase, muscle

Gene Aliases

PK3, TCB, p58, OIP3, PKM2, CTHBP, THBP1, HEL-S-30

Gene ID

5315

Swiss Prot

P11980

Accession Number

NP_002645

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Yeast, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human PKM2

Target

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Partner Proteins

LNX1; NXT2; HUWE1; ISG15; UBC; POLE2; FUS; SUMO2; SUMO3; STAU1; MDM2; SUZ12; EZH2; IPO11; TWF2; PDLIM5; NPLOC4; PLIN3; UBA2; EIF4A3; ANP32A; SRP14; RAD23B; PPP1CA; PIN1; CTPS1; CBS; CAPZA2; ACAT2; NLK; FBXO6; YWHAQ; RAF1; IGSF8; HDAC6; MAPK7; NPM1; CDK2

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

HLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQ

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Sheep: 77%; Yeast: 85%; Zebrafish: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

58kDa

Protein Length

531

NCBI Gene Symbol

PKM

Host or Source

Rabbit

Protein Name

Pyruvate kinase isozymes M1/M2

Gene Name URL

PKM

CAS Number

9007-83-4

Nucleotide Accession Number

NM_002654

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