ARFGAP1 Antibody - middle region
Product Specifications
CAS Number
9007-83-4
Gene Name
ADP-ribosylation factor GTPase activating protein 1
Gene Aliases
ARF1GAP, HRIHFB2281
Gene ID
55738
Swiss Prot
Q8N6T3
Accession Number
NP_060679
Reactivity
Human, Dog
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human ARFGAP1
Target
The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1) . The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.
Partner Proteins
KRTAP10-9; KRTAP9-2; KRTAP3-2; GABARAPL2; GRN; FOS; TRIM23; UBC; RPL22; HK1; RAD52; RPL11; RFC3; PRKACB; APOE; APOC3; APOB; ASB10; RASIP1; RAB23; COPS5; MED27; REEP5; APP; LRRK2; MAP1LC3B; TK1; SMN1; TMED2; CYTH2; COPA; CSNK1D; RGS2; KDELR1; ARF1
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
WRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAE
Applications
WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Dog: 83%; Human: 100%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
45kDa
Protein Length
406
NCBI Gene Symbol
ARFGAP1
Host or Source
Rabbit
Protein Name
ADP-ribosylation factor GTPase-activating protein 1
Gene Name URL
ARFGAP1
Nucleotide Accession Number
NM_018209
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