ARFGAP1 Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

ADP-ribosylation factor GTPase activating protein 1

Gene Aliases

ARF1GAP, HRIHFB2281

Gene ID

55738

Swiss Prot

Q8N6T3

Accession Number

NP_060679

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human ARFGAP1

Target

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1) . The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.

Partner Proteins

KRTAP10-9; KRTAP9-2; KRTAP3-2; GABARAPL2; GRN; FOS; TRIM23; UBC; RPL22; HK1; RAD52; RPL11; RFC3; PRKACB; APOE; APOC3; APOB; ASB10; RASIP1; RAB23; COPS5; MED27; REEP5; APP; LRRK2; MAP1LC3B; TK1; SMN1; TMED2; CYTH2; COPA; CSNK1D; RGS2; KDELR1; ARF1

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

GQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFL

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 93%; Guinea Pig: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

45kDa

Protein Length

406

NCBI Gene Symbol

ARFGAP1

Host or Source

Rabbit

Protein Name

ADP-ribosylation factor GTPase-activating protein 1

Gene Name URL

ARFGAP1

Nucleotide Accession Number

NM_018209

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