ULK1 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

Unc-51-like kinase 1 (C. elegans)

Gene Aliases

ATG1, ATG1A, UNC51, hATG1, Unc51.1

Gene ID

8408

Swiss Prot

O75385

Accession Number

NP_003556

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N-terminal region of Human ULK1

Target

ULK1 is a serine/threonine-protein kinase involved in autophagy in response to starvation. It acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. It is activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. It may phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences and plays a role early in neuronal differentiation and is required for granule cell axon formation.

Partner Proteins

SESN2; SQSTM1; ATG16L1; EXOC8; EXOC2; BECN1; RALB; AMBRA1; ULK1; UBC; TRAF6; MBP; ATG13; MAP1LC3C; MAP1LC3A; MAP1LC3B; GABARAPL1; GABARAPL2; GABARAP; MLST8; RPTOR; MTOR; PPP1CA; ATG4B; CDC37; G3BP1; RB1CC1; USP10; PRKAG1; PRKAB2; PRKAA2; PRKAA1; SYNGAP1

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

PEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTL

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

112 kDa

Protein Length

1050

NCBI Gene Symbol

ULK1

Host or Source

Rabbit

Protein Name

Serine/threonine-protein kinase ULK1

Gene Name URL

ULK1

Nucleotide Accession Number

NM_003565

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

NBP2-22541 500 µg

Mouse Monoclonal ICAM-1/CD54 Antibody (1A29)

Ask

View Details

NBP3-08720MFV500 0.1 mL

Mouse Monoclonal HLA DQ Antibody (rSPV-L3) [mFluor Violet 500 SE]

Ask

View Details

NBP2-98569AF647 0.1 mL

Rabbit Polyclonal SLC44A3 Antibody [Alexa Fluor 647]

Ask

View Details

ELI-51550c 96 Tests

Chicken ZBTB17 ELISA KIT

Ask

View Details