EIF5 antibody - N-terminal region (ARP53443_P050)

Product Specifications

CAS Number

9007-83-4

Gene Name

Eukaryotic translation initiation factor 5

Gene Aliases

EIF-5, EIF-5A

Gene ID

1983

Swiss Prot

P55010

Accession Number

NP_892116

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human EIF5

Target

EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .

Partner Proteins

DUSP12; HUWE1; UBC; TUBB; UBFD1; SCLY; ISOC1; MAT2B; FERMT2; YWHAQ; STK24; YWHAZ; YWHAH; YWHAE; EIF4H; VCL; SNRPD1; PCYT2; PFAS; PDHB; PAFAH1B1; HSPA9; HNRNPK; GNAI3; GLA; G6PD; DFFA; TBCB; CAPNS1; ALDH7A1; LMNA; PUM1; NRGN; FN1; EIF3B; RPL29; APP; Nedd4

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

SVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPT

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

49kDa

Protein Length

431

NCBI Gene Symbol

EIF5

Host or Source

Rabbit

Protein Name

Eukaryotic translation initiation factor 5

Gene Name URL

EIF5

Nucleotide Accession Number

NM_183004

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