MDFI Antibody - middle region (ARP50514_P050)
Product Specifications
CAS Number
9007-83-4
Gene Name
MyoD family inhibitor
Gene Aliases
I-MF, I-mfa
Gene ID
4188
Swiss Prot
Q99750
Accession Number
NP_005577
Reactivity
Human, Cow, Dog, Pig, Rabbit, Sheep
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of Human MDFI
Target
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.
Partner Proteins
PDIA5; USP20; ARID5A; ZNF266; KAT5; SPRY2; SPRY1; NR1H3; ZBTB24; ARHGAP32; KIAA0040; CREB5; MAGED1; ZNF264; GTF3C5; APLN; AKAP17A; ZNF230; ZNF223; ZNF155; ZNF138; ZNF136; ZNF124; ZNF79; WNT11; VHL; NR1H2; TYRO3; TNP2; TNNI1; TGIF1; GNAI2; GATA2; EIF4A2; D
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
AVTCQPQGNPLGCTPLLPNDSGHPSELGGTRRAGNGALGGPKAHRKLQTH
Applications
WB
Purification
Affinity purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Cow: 79%; Dog: 79%; Human: 100%; Pig: 86%; Rabbit: 79%; Sheep: 86%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
27kDa
Protein Length
246
NCBI Gene Symbol
MDFI
Host or Source
Rabbit
Protein Name
MyoD family inhibitor
Gene Name URL
MDFI
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