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PELI2 Antibody - N-terminal region

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Product Specifications

CAS Number

9007-83-4

Gene Name

Pellino E3 ubiquitin protein ligase family member 2

Gene ID

57161

Swiss Prot

Q9HAT8

Accession Number

NP_067078.1

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N-terminal region of Human PELI2

Target

PELI2 is a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. It is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. It may be important for LPS- and IL1B-induced MAP3K7- dependent, but not MAP3K3-dependent, NF-kappa-B activation. And it can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.

Partner Proteins

SRPK1; FRYL; CREB3L3; THAP7; ERO1L; DEAF1; LAMC3; PICALM; LTBP3; IRAK1; HNRNPK; FN1; UBC; UBQLN4; IRAK4; TRAF6; NR2C2; BCL10; MYD88; IRAK2

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

STISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLGEKAAKWKNPDGHMD

Applications

WB

Purification

Affinity purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

46 kDa

Protein Length

420

NCBI Gene Symbol

PELI2

Host or Source

Rabbit

Protein Name

E3 ubiquitin-protein ligase pellino homolog 2

Gene Name URL

PELI2

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