POLR3A Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

Polymerase (RNA) III (DNA directed) polypeptide A, 155kDa

Gene Aliases

ADDH, C160, HLD7, RPC1, WDRTS, RPC155, hRPC155

Gene ID

11128

Swiss Prot

O14802

Accession Number

NP_008986

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human POLR3A

Target

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.

Partner Proteins

SUMO2; UBC; ASB13; NEDD4; POLR3H; VPS18; POLR3B; POLR1D; CRCP; POLR1A; POLR3C; POLR1C; SMARCA5; POLR2H; POLR2E; POLR3D; IKBKG; PCBD1; EWSR1; RPAP2; RPAP3; RPAP1; GPN1; RAE1; POLR3E; PKP2; GTF2B

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

AYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDT

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 79%; Dog: 86%; Guinea Pig: 86%; Horse: 79%; Human: 100%; Mouse: 79%; Rabbit: 86%; Rat: 86%; Yeast: 85%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

156kDa

Protein Length

1390

NCBI Gene Symbol

POLR3A

Host or Source

Rabbit

Protein Name

DNA-directed RNA polymerase III subunit RPC1

Gene Name URL

POLR3A

Nucleotide Accession Number

NM_007055

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