What is the catalog number for PIGA antibody - middle region (ARP45344_P050)?

The catalog number for PIGA antibody - middle region (ARP45344_P050) is ARP45344_P050.

What sizes are available for PIGA antibody - middle region (ARP45344_P050)?

PIGA antibody - middle region (ARP45344_P050) is available in the following size(s): 100 µL.

Is PIGA antibody - middle region (ARP45344_P050) in stock?

PIGA antibody - middle region (ARP45344_P050) is currently made to order / not held in immediate stock. Contact us for lead time.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

PIGA antibody - middle region (ARP45344_P050)

Product Specifications

CAS Number

9007-83-4

Gene Name

Phosphatidylinositol glycan anchor biosynthesis, class A

Gene Aliases

GPI3, PNH1, PIG-A, MCAHS2

Gene ID

5277

Swiss Prot

B3KUV7

Accession Number

NP_065206

Reactivity

Human, Mouse, Rat, Cow, Dog, Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human PIGA

Target

PIGA is a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene.This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized.

Partner Proteins

UBC; ELAVL1; PIGY; DPM2; PIGQ; PIGH; PIGP

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

SVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDR

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 86%; Dog: 93%; Human: 100%; Mouse: 93%; Pig: 79%; Rabbit: 79%; Rat: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

19kDa

Protein Length

169

NCBI Gene Symbol

PIGA

Host or Source

Rabbit

Protein Name

Phosphatidylinositol N-acetylglucosaminyltransferase subunit A Ensembl ENSP00000416102

Gene Name URL