CDC27 Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

Cell division cycle 27 homolog (S. cerevisiae)

Gene Aliases

APC3, HNUC, NUC2, H-NUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E

Gene ID

996

Swiss Prot

P30260

Accession Number

NP_001247

Reactivity

Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human CDC27

Target

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Partner Proteins

ANAPC2; UBE2S; PTTG1; FBXO43; MAD2L1; COMT; CDC20; CCNB1; BUB1B; UBC; BIRC6; ANAPC4; CDH1; FZR1; MIB1; HECW2; ANAPC11; PHF8; CUEDC2; Sirt2; Dlg4; GKN1; ANAPC7; ANAPC5; DCPS; ADNP; MDC1; PTEN; TRIM33; REV1; ANAPC15; VCP; MAD2L2; NOTCH1; PAXIP1; UBE4B; TTK

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

KLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKIST

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Rabbit: 100%; Rat: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

92kDa

Protein Length

824

NCBI Gene Symbol

CDC27

Host or Source

Rabbit

Protein Name

Cell division cycle protein 27 homolog

Gene Name URL

CDC27

Nucleotide Accession Number

NM_001256

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

NBP2-98798PE 0.1 mL

Rabbit Polyclonal FXR1 Antibody [PE]

Ask

View Details

MR215461L4V 200 µL

Mark4 (NM_172279) Mouse Tagged ORF Clone Lentiviral Particle

Ask

View Details

E053438 1 Each

Mouse FFAR2 ELISA Kit

Ask

View Details

AFG-ELL-0079 > 1x 10^6 Cells / Vial

Human Breast Cancer Cells [ZR-75-1]

Ask

View Details

GTR15417426 1 Vial

Human ARHGEF18 Knockout HEK293T cell line

Ask

View Details

RC202068 10 µg

SNAP25 (NM_003081) Human Tagged ORF Clone

Ask

View Details