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ANAPC5 Antibody - middle region (ARP43211_P050)

Selected Variant

ARP43211_P050

Size: 100 µL

Product Specifications

CAS Number

9007-83-4

Gene Name

Anaphase promoting complex subunit 5

Gene Aliases

APC5

Gene ID

51433

Swiss Prot

Q9UJX4-2

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of Human ANAPC5

Target

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Partner Proteins

ANAPC2; UBE2S; UBC; ANAPC4; HECW2; LATS2; PHF8; SOX2; PTEN; CDC27; BACH1; TRIM33; ANAPC7; ANAPC15; CDH1; CDC20; PAXIP1; MDC1; TP53BP1; NOTCH1; ANAPC1; ANAPC13; CDC16; CDC23; FBXO5; USP37; CUL4A; SH3KBP1; E2F1; SMARCAD1; MAPK6; Gm9174; Anapc5; Mad2l1; Anap

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

HSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYTSPP

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

24kDa

Protein Length

219

NCBI Gene Symbol

ANAPC5

Host or Source

Rabbit

Protein Name

Anaphase-promoting complex subunit 5

Gene Name URL

ANAPC5

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