NEDD4L Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

Neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

Gene Aliases

RSP5, PVNH7, NEDD4-2, NEDD4.2, hNEDD4-2

Gene ID

23327

Swiss Prot

Q96PU5-5

Accession Number

NP_056092

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L

Target

NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.

Partner Proteins

NEDD4L; DAZAP2; UBE2D1; PTCH1; WNK3; TNK2; SNCA; SLC9A3; SMAD6; KCNH2; HCN1; SMAD3; UBE2N; SNA3; NOTCH1; UBE2D3; UBC; SGK1; KCNQ1; SGK3; GORAB; MED28; FBXL15; vpr; RNF11; AATF; YWHAQ; LAPTM5; SCNN1B; SMAD2; ARRDC3; ARRDC4; UBE2L3; UBE2D2; SMAD7; UBE2K; UB

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

TVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLP

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

110 kDa

Protein Length

955

NCBI Gene Symbol

NEDD4L

Host or Source

Rabbit

Protein Name

E3 ubiquitin-protein ligase NEDD4-like

Gene Name URL

NEDD4L

Nucleotide Accession Number

NM_015277

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