Btrc antibody - C-terminal region (ARP42160_P050)

Product Specifications

CAS Number

9007-83-4

Gene Name

Beta-transducin repeat containing protein

Gene Aliases

Sl, HOS, FWD1, Fbw1, Fbw1a, Slimb, b-TrCP, beta-T, Beta-Tr, beta-TrCP, Beta-Trcp1, SCF b-TRCP, E3RSIkappaB, E3RS-IkappaB

Gene ID

12234

Swiss Prot

Q3ULA2

Accession Number

NP_001032847

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Target

Btrc is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It recognizes and binds to phosphorylated target proteins. SCF (BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF (BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF (BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF (BTRC) mediates the ubiquitination of phosphorylated SNAI1.

Partner Proteins

ZRANB1; Tpx2; Taz; Per2; Cul1; Skp1a; Reg1; Stat1; Nfe2l2; Per1; Htt; Cops2; Ikbkb; Nkx3-2; Cdc25b; Nfkbia; Gli2

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

LVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAHAEPPRSPSRTY

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol