MGMT Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

O-6-methylguanine-DNA methyltransferase

Gene ID

4255

Swiss Prot

P16455

Accession Number

NP_002403

Reactivity

Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Yeast

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human MGMT

Target

MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Partner Proteins

UBC; BRCA2; ANXA2; ANXA1; SLC25A6; SLC25A4; ACTB; CCNB1; FBL; EEF1B2; EEF1A2; EEF1A1; DDX5; CDKN1A; CDK2; CDK1; H2BFM; FBXO27; TUBA1C; ANP32E; IL25; DDX24; CDK12; FBXO2; USP19; TUBB4B; PRPF4; RNF8; EIF3H; CDK10; HIST1H4A; HIST1H2AM; HIST3H3; SMC1A; YWHAZ

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

ISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGG

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 79%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

22kDa

Protein Length

207

NCBI Gene Symbol

MGMT

Host or Source

Rabbit

Protein Name

Methylated-DNA--protein-cysteine methyltransferase

Gene Name URL

MGMT

Nucleotide Accession Number

NM_002412

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