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U2AF2 Antibody - C-terminal region

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Product Specifications

CAS Number

9007-83-4

Gene Name

U2 small nuclear RNA auxiliary factor 2

Gene Aliases

U2AF65

Gene ID

11338

Swiss Prot

Q80XR5

Accession Number

NP_001012496

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Yeast, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human U2AF2

Target

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.

Partner Proteins

THAP1; PUF60; U2AF1; SNRPA; AES; DACH1; SUMO3; UBC; SUZ12; EED; TERF1; EZH2; RNF2; PRPF40A; TCERG1; APBB1; PIN1; SH3RF2; SRPK2; SRPK1; SF1; SRSF1; RBM39; QKI; SCAF11; PRPF3; RBM10; RBM4; SMAD5; IL7R; HNRNPK; HNRNPD; HLA-DMA; SUGP1; CCAR1; SAP30BP; RBFOX2

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

TEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGV

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Yeast: 79%; Zebrafish: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

53kDa

Protein Length

471

NCBI Gene Symbol

U2AF2

Host or Source

Rabbit

Protein Name

U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2, isoform CRA_a EMBL EDL31286.1

Gene Name URL

U2AF2

Nucleotide Accession Number

NM_001012478

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