PRMT5 Antibody - N-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

Protein arginine methyltransferase 5

Gene Aliases

HSL7, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5

Gene ID

10419

Swiss Prot

O14744

Accession Number

NP_001034708

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT5

Target

PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs) . It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.

Partner Proteins

UBC; TP53; ING2; PRDM1; NFE4; WDR5; SUMO2; WWOX; CDYL2; LENG8; LDHAL6B; PRPF38A; ING5; ZNF436; WDR77; GRHL3; TRIB3; RSRP1; LUC7L; RBM23; GTPBP2; H3F3C; FOXK1; ZUFSP; FAM76B; NUDCD2; MAGEB2; NELFCD; COPS4; COPS7A; DCAF8; SPAG8; SLU7; MCRS1; CUL4A; ZNF2; YW

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Sequence

FDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLS

Applications

IHC, WB

Purification

Protein A purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol