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TIMELESS antibody - C-terminal region (ARP38404_T100)

Product Specifications

Gene Name

Timeless homolog (Drosophila)

Gene Aliases

TIM, TIM1, hTIM

Gene ID

8914

Swiss Prot

Q9UNS1

Accession Number

NP_003911

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Sheep, Yeast

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human TIMELESS

Target

The human Timeless protein interacts with both the circadian clock protein cryptochrome 2 and with the cell cycle checkpoint proteins Chk1 and the ATR-ATRIP complex and plays an important role in the DNA damage checkpoint response. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein.

Partner Proteins

UBC; TUT1; TF; XRCC1; FKBP3; SP1; SUMO2; Tipin; Timeless; ZSCAN1; PCBD1; CRY1; PER2; PER1; CLSPN; PCNA; per; Clock; ATRIP; CRY2; CHEK1

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

EEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRY

Applications

WB

Purification

Protein A purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1.0 mg/ml

Homology

Cow: 100%; Dog: 93%; Guinea Pig: 83%; Horse: 83%; Human: 100%; Mouse: 90%; Rat: 100%; Sheep: 100%; Yeast: 91%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

139kDa

Protein Length

1208

NCBI Gene Symbol

TIMELESS

Host or Source

Rabbit

Protein Name

Protein timeless homolog

Gene Name URL

TIMELESS

CAS Number

9007-83-4

Nucleotide Accession Number

NM_003920

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