SPI1 Antibody - middle region

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Product Specifications

CAS Number

9007-83-4

Gene Name

Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Gene Aliases

OF, PU.1, SFPI1, SPI-1, SPI-A

Gene ID

6688

Swiss Prot

P17947

Accession Number

NP_003111

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human SPI1

Target

SPI1 is known to collaborate with multiple transcription factors and to regulate expression of immunoglobulin genes. Lack of SPI1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Partner Proteins

HOXA10; TMX1; VAV1; IRF1; IRF8; GATA3; GATA2; GATA1; GFI1; TBP; RB1; BYRF1; RARA; PML; HDAC3; SKI; SIN3A; HDAC1; BCL6; CEBPA; PIP; SPIB; SSRP1; IRF4; KAT6A; CEBPE; NFATC1; CREBBP; NONO; CEBPB; JUN; ERG; NFKB1; MITF; FUS; CREM; ATF1; CEBPD; MECP2; DNMT3B

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

QKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAER

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 85%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

31kDa

Protein Length

270

NCBI Gene Symbol

SPI1

Host or Source

Rabbit

Protein Name

Transcription factor PU.1

Gene Name URL

SPI1

Nucleotide Accession Number

NM_003120

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