Human Vinculin / VCL Protein, His Tag
Human Vinculin, His Tag (VIN-H52H9) is expressed from human 293 cells (HEK293) . It contains AA Pro 2 - Gln 1066 (Accession # P18206-2) .
Product Specifications
Background
Vinculin is a ubiquitously expressed actin-binding protein and involves in cross-linking of the cell cytoskeleton with the extracellular matrix for mechanical support of the cell. Vinculin is frequently used as a marker for both cell-cell and cell-extracellular matrix (focal adhesion) adherens-type junctions.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 118.5 kDa. The protein migrates as 130-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P18206-2
Expression Region
Pro 2 - Gln 1066
Host
HEK293
Target
Vinculin
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Human Vinculin, His Tag (Cat. No. VIN-H52H9) at 1 μg/mL (100 μL/well) can bind Vinculin Antibody with a linear range of 0.2-16 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
118.5 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
1mg*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/VIN-H52H9.pdf
Species
Human
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