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Atf7ip Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

Activating transcription factor 7 interacting protein

Gene Aliases

A, AM, Mcaf, Mcaf1, 2610204M12Rik, 5830415B17Rik

Gene ID

54343

Swiss Prot

Q7TT18

Accession Number

NP_062299

Reactivity

Mouse, Rat, Pig

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of Mouse Atf7ip

Target

Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Atf7ip can both act as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Atf7ip is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3) . The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) . Atf7ip may have ATPase activity.

Partner Proteins

Atf7; Gtf2f1; Gtf2e2; Aagab; Ccnh; Gtf2h1; Ercc3; Cdk7; C1qbp; POLR2H; POLR2G; POLR2D; POLR2C

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

MSTPEGEKSEKDGKAEEEERVPAEEQPPVRNEFSRRKRSKSEDMDSVESK

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Mouse: 100%; Pig: 92%; Rat: 85%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

138kDa

Protein Length

1306

NCBI Gene Symbol

ATF7IP

Host or Source

Rabbit

Protein Name

Activating transcription factor 7-interacting protein 1

Gene Name URL

Atf7ip

Nucleotide Accession Number

NM_019426

Frequently Asked Questions

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