Dnmt1 antibody - N-terminal region (ARP37033_P050)
Product Specifications
CAS Number
9007-83-4
Gene Name
DNA methyltransferase (cytosine-5) 1
Gene Aliases
MTa, Dnmt, MCMT, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD, m.MmuI, MommeD2
Gene ID
13433
Swiss Prot
P13864
Accession Number
NP_034196
Reactivity
Mouse
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of mouse Dnmt1
Target
Dnmt1 methylates CpG residues.Dnmt1 preferentially methylates hemimethylated DNA. Dnmt1 associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance.Dnmt1 associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones.Dnmt1 mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, Dnmt1 is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Partner Proteins
Uhrf1; Parp1; Eed; hist1h3g; DSK2; VPS9; Kat5; Ubc; Atm; NUB1; UBQLN1; SQSTM1; PSMD4; NBR1; RAD23B; Hesx1; Ezh2; Zbtb16; Cul3; Cdh1; Dmap1; Tcf3; hist1h4a; hist1h2bj; hist2h2ab; Dnmt1; Kcnq1ot1; Pla2g2a; Apc; Tox3; Rela; HDAC1
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
SSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKR
Applications
WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Mouse: 100%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
183kDa
Protein Length
1619
NCBI Gene Symbol
DNMT1
Host or Source
Rabbit
Protein Name
DNA (cytosine-5) -methyltransferase 1
Gene Name URL
Dnmt1
Nucleotide Accession Number
NM_010066
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