Eed Antibody - N-terminal region
Product Specifications
CAS Number
9007-83-4
Gene Name
Embryonic ectoderm development
Gene Aliases
L7, l (7), lusk, l7Rn5, l (7) 5Rn
Gene ID
13626
Swiss Prot
Q921E6
Accession Number
NP_068676
Reactivity
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Target
Eed is a polycomb group (PcG) protein. It is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Eed plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Eed is required for stable maintenance of XCI in both embryonic and extra-embryonic tissues. Eed may prevent transcriptional activation of facultative heterochromatin during differentiation. Eed is required for development of secondary trophoblast giant cells during placental development. Eed may regulate hippocampal synaptic plasticity in the developing brain.
Partner Proteins
Ezh2; Suz12; Rab3gap1; Ddx47; Nop10; Nip7; Bola2; Tmem14c; Uqcr10; Eif3f; Cbwd1; Smc5; Etf1; Svil; Fbxo11; Srsf7; Safb; Safb2; Abcf1; Bag6; C330027C09Rik; Pak2; Pphln1; Ttll12; Sun2; Tomm22; Eif3l; E430025E21Rik; Eny2; Dcaf13; Trio; Ccar2; Fermt2; Vps41
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
AKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKS
Applications
WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
50kDa
Protein Length
441
NCBI Gene Symbol
EED
Host or Source
Rabbit
Protein Name
Polycomb protein EED
Gene Name URL
Eed
Nucleotide Accession Number
NM_021876
Curated Selection
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