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UBTF antibody - middle region (ARP33480_P050)

Product Specifications

CAS Number

9007-83-4

Gene Name

Upstream binding transcription factor, RNA polymerase I

Gene Aliases

UBF, UBF1, UBF2, UBF-1, CONDBA, NOR-90

Gene ID

7343

Swiss Prot

P17480

Accession Number

NP_055048

Reactivity

Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human UBTF

Target

UBTF is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1. Two UBTF polypeptides, of 94 and 97 kD, exist in the human. UBTF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxesUpstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs') . Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]) . UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]) .[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Partner Proteins

UBC; MDM2; RNF2; Dlg4; ESR1; CDK4; CDK2; CCNE1; CCND1; WDR70; YEATS4; RB1; GRK5; KAT5; SIRT7; POLR1A; MYBBP1A; HDAC1; RUNX2; SUMO2; SMARCA5; MAPK3; MAPK1; SUMO1; PIK3CA; IRS1; TAF1D; tat; MCRS1; TLE1; ACTN4; POLR1E; CHGB; SMURF1; TCOF1; MAD1L1; ZMYM2; SER

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

LESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPV

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 86%; Guinea Pig: 79%; Horse: 77%; Human: 100%; Mouse: 93%; Pig: 86%; Rabbit: 93%; Rat: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

89kDa

Protein Length

764

NCBI Gene Symbol

UBTF

Host or Source

Rabbit

Protein Name

Nucleolar transcription factor 1

Gene Name URL

UBTF

Nucleotide Accession Number

NM_014233

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