POLR2B antibody - N-terminal region (ARP32457_T100)

Product Specifications

CAS Number

9007-83-4

Gene Name

Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa

Gene Aliases

RPB2, POL2RB, hRPB140

Gene ID

5431

Swiss Prot

P30876

Accession Number

NP_000929

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2B

Target

POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Partner Proteins

ESR1; UBC; TCEB3; SRC; PSMB9; SUMO2; XRN2; WWOX; SUZ12; RNF2; NEDD4; ITCH; RABEP2; HECW2; FBXO6; CDK20; HDAC11; ILK; POLR2C; MED19; MED26; RECQL5; VCP; BARD1; MDC1; POLR3F; TUBB4B; TUBA4A; POLR2I; POLR2E; POLR2D; POLR2A; DCTN1; ATP6V1B2; MEN1; CDK7; CCNH

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

TYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTD

Applications

Purification

Protein A purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1.0 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

134kDa

Protein Length

1174

NCBI Gene Symbol

POLR2B

Host or Source

Rabbit

Protein Name

DNA-directed RNA polymerase II subunit RPB2

Gene Name URL

POLR2B

Nucleotide Accession Number

NM_000938

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