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SMAD5 Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

SMAD family member 5

Gene Aliases

DWFC, JV5-1, MADH5

Gene ID

4090

Swiss Prot

Q99717

Accession Number

NP_001001420

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human SMAD5

Target

SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.

Partner Proteins

UBC; SUMO1; HECW2; HBG2; BCAT1; SOX5; SNRPA; SNRNP70; SFPQ; RYR2; RBM4; PTPN12; PSMD11; NEDD4; SMAD4; SMAD1; ANKRD13A; PPIL4; SOX7; SMURF2; SMURF1; MTMR10; CHMP3; CXXC5; SF3B1; U2AF2; PNKP; WWP1; NAA20; EPN1; SMAD3; SMAD2; PAX6; COPS5; HGS; EP300; ELAVL1

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

YPPSPASSTYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMD

Applications

WB

Purification

Protein A purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1.0 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 93%; Zebrafish: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

51kDa

Protein Length

465

NCBI Gene Symbol

SMAD5

Host or Source

Rabbit

Protein Name

Mothers against decapentaplegic homolog 5

Gene Name URL

SMAD5

Nucleotide Accession Number

NM_001001420

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