SMAD4 Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

SMAD family member 4

Gene Aliases

JIP, DPC4, MADH4, MYHRS

Gene ID

4089

Swiss Prot

Q13485

Accession Number

NP_005350

Reactivity

Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human SMAD4

Target

SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction

Partner Proteins

RASSF5; LMO4; SMAD3; SMAD2; SKIL; UBC; NEDD8; SUMO1; SMURF2; SMURF1; TRIM33; CRYAB; COPS5; NLK; OTUB1; SMAD9; UBE2I; FHL3; FHL2; FHL1; CSNK1D; TGFB1I1; SMAD1; NCOR1; SKI; KLF5; IRF7; IRF3; ZNF521; ZNF423; SOX12; SNRNP70; RPL28; RFX1; RELA; RALA; PSMD11; P

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

NIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNST

Applications

Purification

Protein A purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1.0 mg/ml

Homology

Cow: 100%; Dog: 92%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100%; Sheep: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

60 kDa

Protein Length

552

NCBI Gene Symbol

SMAD4

Host or Source

Rabbit

Protein Name

Mothers against decapentaplegic homolog 4

Gene Name URL

SMAD4

Nucleotide Accession Number

NM_005359

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