Elf3 Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

E74-like factor 3 (ets domain transcription factor, epithelial-specific)

Gene Aliases

ERT, ESX, EPR-1, ESE-1

Gene ID

1999

Swiss Prot

P78545

Accession Number

NP_004424

Reactivity

Human, Cow, Dog, Horse, Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human Elf3

Target

ELF3 is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family. ELF3 contains two putative DNA binding domains: an ETS domain, which is unique in that the 5' half shows relatively weak homology to known ets factors, and an A/T hook domain, found in HMG proteins and various other nuclear factors. In contrast to any known ets factors, ELF3 is expressed exclusively in epithelial cells. ELF3 is expected to be a critical regulator of epithelial cell differentiation. ETS factor ELF3 is a transcriptional regulator of angiopoietin-1 gene regulation in the setting of inflammation. ELF3 overexpressed early during human breast tumorigenesis. ELF3 expression confers a transformed and in vitro metastatic phenotype to otherwise normal MCF-12A cells. ELF3 mediates the expression of TGF-beta RII, and the transcriptional inhibition of ets-related transcription factor could be a one of the mechanisms of colonic carcinogenesis. ELF3 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP.

Partner Proteins

MED23; XRCC5; RPL6; XRCC6; EP300; CBR3; Jun; MYC; BTRC; UBC; PAK1; TAF9; SUMO2; TBP; EPS15; CREBBP; NFKB1; EGFR; ERBB2; EHF; GANAB; ISG15; TARS; P4HB; KRT82; KRT31; JAK1; EEF1D; DDX3X; CALR; ATIC

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

GSCGAGAPSPGSSDVSTAGTGASRSSHSSDSGGSDVDLDPTDGKLFPSDG

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol