NFYA Antibody - C-terminal region

Product Specifications

CAS Number

9007-83-4

Gene Name

Nuclear transcription factor Y, alpha

Gene Aliases

HAP2, CBF-A, CBF-B, NF-YA

Gene ID

4800

Swiss Prot

P23511

Accession Number

NP_002496

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human NFYA

Target

NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

Partner Proteins

LUC7L2; POGZ; NFYB; GTF2A1; CSNK2A1; UBC; APPBP2; TP63; TP53; NFYC; EP300; CDK2; CCNA2; CDC25A; APP; RPS6KA6; JUN; HDAC1; ATF2; SP1; TAF6; TAF11; TAF12; PAPOLG; PWP1; SREBF2; ZHX2; ZHX1; ZHX3; KPNB1; SRSF1; HMGA1; SRF; USF2; USF1; NFYA; NR5A1; POU2F1; CEB

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

YLHESRHRHAMARKRGEGGRFFSPKEKDSPHMQDPNQADEEAMTQIIRVS

Applications

IHC, WB

Purification

Protein A purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1.0 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 80%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

37kDa

Protein Length

347

NCBI Gene Symbol

NFYA

Host or Source

Rabbit

Protein Name

Nuclear transcription factor Y subunit alpha

Gene Name URL

NFYA

Nucleotide Accession Number

NM_002505

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