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NFYB Antibody - middle region

Product Specifications

CAS Number

9007-83-4

Gene Name

Nuclear transcription factor Y, beta

Gene Aliases

HAP3, CBF-A, CBF-B, NF-YB

Gene ID

4801

Swiss Prot

P25208

Accession Number

NP_006157

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Yeast

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human NFYB

Target

NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

Partner Proteins

AGTRAP; POLE4; DRAP1; NFYA; LYN; CDK2; CCNE1; CCNA2; TP53; SMYD3; NFYC; MAOB; UBC; CREBBP; SP1; HDAC1; EP300; KAT2B; TP73; TAF12; TAF11; TAF6; GRB2; YBX3; CEBPZ; C1QBP; TBP; CIITA; CNTN2; MYC; ELF1; YBX1

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

EAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYT

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 100%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Yeast: 91%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

23kDa

Protein Length

207

NCBI Gene Symbol

NFYB

Host or Source

Rabbit

Protein Name

Nuclear transcription factor Y subunit beta

Gene Name URL

NFYB

Nucleotide Accession Number

NM_006166

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