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HIF1A Antibody - N-terminal region

Product Specifications

Gene Name

Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Gene Aliases

HIF1, MOP1, PASD8, HIF-1A, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF-1-alpha

Gene ID

3091

Swiss Prot

Q16665

Accession Number

NP_851397

Reactivity

Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human HIF1A

Target

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . HIF1A is the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Partner Proteins

HDAC1; EP300; ARNTL; HDAC3; VHL; UBC; SUMO3; EGLN3; HIF1AN; SENP3; HSP90AA1; ATM; ARNT2; SART3; SUMO2; SIN3A; TRIM28; HSPA4; TRAF6; GNB2L1; EGLN1; SQSTM1; SUMO1; HEXIM1; LAMP2; HSPA8; RB1; SP1; SMAD3; MAFK; PCGF2; TCEB2; TCEB1; MAFG; HSP90AB1; DCUN1D1; RB

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

EGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVS

Applications

IHC, WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 87%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

93 kDa

Protein Length

826

NCBI Gene Symbol

HIF1A

Host or Source

Rabbit

Protein Name

Hypoxia-inducible factor 1-alpha

Gene Name URL

HIF1A

CAS Number

9007-83-4

Nucleotide Accession Number

NM_181054

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